Fig. 1
Screening and profiling of tRFs in the tissues and plasma exosomes from CRC patients and healthy controls. a Schematic presenting the experimental procedure of our study. b Volcano plot showing the gene expression profile of CRC tissues and matching adjacent normal tissues. Down-regulated genes are indicated in green; upregulated genes are indicated in red. c Gene set enrichment analysis of the differential gene in tissues. d A heatmap showing 33 differentially expressed tRFs in 10 paired CRC tissues and matching adjacent normal tissues. e A heatmap showing 25 differentially expressed tRFs between 10 plasma exosomes of CRC and healthy controls. f Volcano plot showing significant DEGs in tissues between the high tRF_group and low tRF_group. Down-regulated genes are indicated in green; upregulated genes are indicated in red. g GSEA plots for high tRF_group and low tRF_group in tissues. h Volcano plot showing significant DEGs in plasma exosomes between the high tRF_group and low tRF_group. Down-regulated genes are indicated in green; upregulated genes are indicated in red. i GSEA plots for high tRF_group and low tRF_group in plasma exosomes. j Percentage of each type of tRFs in tissues. k Percentage of each type of tRFs in plasma exosomes. l Correlation analysis of tRF-3022b, tRF-3030b and tRF-5008b between tissues and plasma exosomes. (***P < 0.001)