Fig. 3
Basal levels of miR-455-5p negatively correlate with cisplatin resistance in vitro. A Heatmap of cell viability values (median normalized) of NSCLC cell lines at increasing concentrations of cisplatin. Heatmap square represents an individual drug concentration drug. For each dataset, the number of cell lines and concentration range used (minimum–maximum) are indicated. B Dose–response curves of the indicated NSCLC cell lines treated with cisplatin for 72 h. Error bars indicate SEM (N = 3 to 5). C Distribution of potency (IC50) versus efficacy values (Emax) of cisplatin in the indicated NSCLC cell lines. Data are mean ± SEM (N = 3 to 5). D Upper panel: bubble plot reporting correlation coefficient (r) between basal level of normalized miRNA expression (Cqn) and IC50 or Emax values. The size of the bubble is proportional to statistical significance calculated by the Spearman correlation test, while colors indicate r coefficient. Yellow: common differentially expressed miRNAs in both EBUS and MED samples; green: miRNAs differentially expressed in EBUS samples only. Lower panel: box plot representing the expression levels (Cqn) of miRNAs in the panel of NSCLC cell lines. E Heatmap of mean value of IC50, Emax and miR-455-5p expression (Cqn) in the indicated cell lines. F Box plot showing miR-455-5p expression levels (Cqn) in chemoresistant (pN2) and chemoresponsive (pN0) patients in MED and EBUS samples. P values were calculated by the Mann–Whitney U test