Fig. 6

BACH1 suppresses MLLr AML progression. A Western blot analysis of indicated proteins in sorted GFP⁺RFP⁺ AML cells infected with EV and Bach1. The asterisk represents a nonspecific band. B Flow cytometry plots (left) and the percentage of GFP⁺RFP⁺ cells in PBMC (right) from the secondary recipients (n = 6). C, D Flow cytometry plots (left) and the percentage of GFP⁺RFP⁺ cells (C) or MKs (D) in BM from the secondary recipients (right, n = 6). E Representative images of Giemsa-Wright staining for EV and Bach1 BM cells upon the second transplantation. Quantification of the frequencies of blast cells was shown on the right. Red and green arrows point to representative blast cells and differentiated cells, respectively. F, G Gross pathology (left) and relative weights (right) (F), hematoxylin–eosin staining (G) of the livers and spleens from the secondary recipients (n = 6). H Survival curves for recipients receiving EV and Bach1 GFP⁺RFP⁺ cells upon secondary transplantation (n = 7). I, J Cell-cycle (I) or apoptosis (J) analysis of GFP⁺RFP⁺ cells in BM from the secondary recipients (n = 7–8). Error bars denote mean ± SD. Statistical significance was determined by two-tailed unpaired t test (B–F, and I, J) or log-rank test (H), and the P values were shown. All animal experiments were repeated at least twice with similar results