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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: Broadly applicable TCR-based therapy for multiple myeloma targeting the immunoglobulin J chain

Fig. 3

Killing of MM cell line U266 by Jchain-specific T-cell clones. A Killing of JCHAIN expressing U266 cells transduced with HLA-A1 or A24 by Jchain A1 and A24 specific T-cell clones in a 6-h 51Cr release assay (top graphs). Killing of WT (HLA-A3 positive) or HLA-A11-transduced U266 cells by Jchain HLA-A3 or A11-specific T-cell clones (bottom graphs). Killing assays were performed using E/T ratios 10:1 and 1:1. Allo-HLA T-cell clones, recognizing peptides derived from housekeeping proteins in specific HLA alleles, were included as positive controls. Average values and standard deviations of technical triplicates are shown. B IFN-γ production measured by ELISA after overnight co-culture using the same target cells as in A in an E/T of 1:6. Values and means of technical duplicates are shown

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