Fig. 5
From: Tumor cell integrin β4 and tumor stroma E-/P-selectin cooperatively regulate tumor growth in vivo
Depletion of ITGB4 reduces proliferation at low confluence, impairs three-dimensional colony forming capacity and enhances apoptosis in vitro. Extinction values in colorimetric proliferation XTT assays at high cell density in conventional cell culture (A). Cell counts per mL on day six after low-density seeding in conventional cell culture (B). Diameters of 3D tumor spheroids after eleven days of growth in soft agar in the presence of standard cell culture media or conditioned media (CM) taken from shControl or shITGB4 cell cultures (C). Percentage of early (Q4) and late (Q2) apoptotic cells in 3D tumor spheroids developed after three days in poly-HEMA-treated cell culture flasks (D). Luminescence and fluorescence measurements indicating the amount of apoptotic, necrotic and viable cells at indicated time points after cultivation in soft agar containing laminin V (LN V, laminin-α3β3γ2) (E). Bar charts represent mean + SD of n = 3. Lines in scatter plots indicate mean values. *p < 0.05; **F < 0.01; ***p < 0.001