Fig. 7
From: Tumor cell integrin β4 and tumor stroma E-/P-selectin cooperatively regulate tumor growth in vivo
ITGB4 depletion enhances leukocyte attraction via chemokine release. Illustration of the experimental setup and quantification of invaded human macrophages per invasive front in a 3D collagen I matrix mixed with standard cell culture media or conditioned media (CM) taken from shControl or shITGB4 cell cultures, each supplemented with M-CSF (A). Concentrations of indicated chemokines in the supernatant of 1 × 106 control or ITGB4 KD cells of the indicated cell lines (B). Number of microvesicles per mL and microvesicle size in supernatants of control or ITGB4 KD TC cultures (C). Concentrations of indicated chemokines in the supernatant of control or ITGB4 KD cells of the indicated cell lines with or without treatment with STAT inhibitors as indicated (D). Black lines in scatter plots indicate mean values. Bar charts represent mean + SD of n ≥ 3. *p < 0.05; **p < 0.01; ***p < 0.001