Fig. 8
From: Tumor cell integrin β4 and tumor stroma E-/P-selectin cooperatively regulate tumor growth in vivo
ITGB4 knockdown and E-/P-selectin knockout cooperatively determine the local and systemic immune environment in the early phase of tumor establishment. Tumor weights on d10 after s. c. injection of PC-3 control vs. ITGB4 knockdown cells into E-/P-selectin wildtype vs. knockout mice and representative density plots of the ex vivo flow cytometric analysis of s. c. tumor nodule homogenates (A). Fractions of leukocyte subpopulations characterized by indicated surface molecules and granularity (SSC) in the s. c. tumor nodules (A) and corresponding blood (B), spleen (C), and bone marrow (D) samples. For representative flow cytometry density plots of blood, spleen and bone marrow samples, please see Additional file 6: Fig. S6. Representative photomicrographs of HE- and anti-Ly6G (Gr-1 epitope)-stained spleen samples and corresponding spleen weights of s. c. tumor nodule-bearing mice (d10) (C, the arrow indicates a megakaryocyte, arrowheads indicate immature granulocytes with ring-shaped nuclei, see Additional file 7: Fig. S7 also). Black lines in scatter plots in (A)–(D) represent mean values. *p < 0.05; **p < 0.01; ***p < 0.001