Fig. 4

CAR T-cell expansion and persistence. A Kinetics of CAR T cells in peripheral blood of individual patients, as measured by flow cytometry; the red dotted line indicates the blank staining upper limit (blank staining with isotype control antibody were included in each sample analysis, and ranged from 0 to 0.02%); data were recorded until the cut-off date or the time point that discontinued follow-up; different types of symbols indicate different patients, and lines of different colors indicate different subgroups. B Kinetics of CAR vector transgene copies per microgram of genomic DNA of peripheral blood mononuclear cells in individual patients, as measured by quantitative PCR; the dotted line indicates the lower limit of quantitation for this assay; data were recorded until the cut-off date or the time point that discontinued follow-up; different types of symbols indicate different patients, and lines of different colors indicate different groups. C–F The peak numbers of CAR T cells in the peripheral blood of the 12 patients without SCT consolidation according to long-term response (C) or adverse events (D–F). G CAR T-cell persistence determined by flow cytometry according receiving haploidentical or MSD/MUD cells. The longest horizontal bars indicate the median values, the gray and light orange boxes indicate values that range from 25 to 75th percentile, different color of symbols indicate patients from different subgroups, and the whiskers extend from the minimum to the maximum values. The P value is based on unpaired student’s t-tests. CAR—chimeric antigen receptor; PCR—polymerase chain reaction; SCT—stem cell transplantation; MSD—matched sibling donor; MUD—matched unrelated donor; GVHD—graft-versus-host disease