Fig. 3
LCN2 overexpression promotes tissue ferroptosis and wasting. A, B. 3T3-L1 cells were treated with LCN2 for 24 h. A. Representative immunofluorescence staining of lipid peroxidation. Scale bars, 50 μm. B. qPCR analysis of mRNA levels of the indicated ferroptosis-related genes in 3T3-L1 cells. n = 3 per group. C–J. Mice were injected intravenously with the control or LCN2-overexpressing lentivirus, and tissues were harvested on day 21. C. qPCR measurements of Lcn2 mRNA levels in the eWAT, iWAT, and Gast. n = 5–6 per group. D. MDA concentrations in the eWAT, iWAT, and Gast, assessed using chemiluminescence. n = 5–6 per group. E. ELISA evaluation of serum LCN2 concentrations. n = 6 per group. F. Fe2+ concentrations in eWAT, iWAT, and Gast, assessed by chemiluminescence. n = 5–6 per group. G. Representative images of mice injected with the control or LCN2-overexpressing lentivirus. Body weights of mice injected with the control or LCN2-overexpressing lentivirus. n = 5 per group. H. Representative image of the eWAT, iWAT, and Gast from mice injected with the control or LCN2-overexpressing lentivirus. I. Weights of the eWAT, iWAT, and Gast isolated from mice injected with the control or LCN2-overexpressing lentivirus. n = 5 per group. J. Representative H&E staining of the iWAT, eWAT, and Gast from mice injected with the control or LCN2-overexpressing lentivirus. Scale bars, 100 μm. Data are shown as the mean ± SEM. Statistical analyses were performed using the unpaired Student’s t-tests (B–E, F, G, I)