Fig. 6

LCN2 knockout reduces tissue ferroptosis and wasting in lung cancer cachexia. A–G. Lcn2^f/+;LysM^cre and Lcn2^f/f;LysM^cre mice were injected with LLC cells to induce the lung cancer cachexia model, and the tissues were harvested on day 21. A. Representative images and body weights of Lcn2^f/+;LysM^cre and Lcn2^f/f;LysM^cre lung cancer cachexia model mice and their respective controls. Representative images of eWAT, iWAT (B), and Gast (C) from the Lcn2^f/+;LysM^cre and Lcn2^f/f;LysM^cre lung cancer cachexia model mice and controls. n = 5–6 per group. D. Weights of the eWAT, iWAT, and Gast of Lcn2^f/+;LysM^cre and Lcn2^f/f;LysM^cre lung cancer cachexia model mice and controls. n = 5–6 per group. E. Representative images of the iWAT, eWAT, and Gast of Lcn2^f/+;LysM^cre and Lcn2^f/f;LysM^cre lung cancer cachexia model mice and controls. H&E staining; scale bars, 100 μm. Analysis of (F) Fe²⁺ concentration, (G) MDA concentration in the eWAT, iWAT, and Gast of Lcn2^f/+;LysM^cre and Lcn2^f/f;LysM^cre lung cancer cachexia model mice and controls. n = 5–6 per group. H–M. Lung cancer cachexia model mice were injected with 50 μg IgG or anti-LCN2 antibody every 3 days between days 4 and 20, and the tissue samples were harvested on day 21. H. Representative images (left) and body weights (right) of control mice, neutrophil-depleted lung cancer cachexia model mice, and control IgG-injected lung cancer cachexia model mice. n = 5 per group. I. Representative images (left) and weights (right) of the eWAT. n = 5 per group. J. Representative images (left) and weights (right) of the iWAT. n = 5 per group. K. Fe²⁺ (n = 5–6 per group) concentrations in the eWAT, iWAT, and Gast. L. MDA (n = 6 per group) concentrations in the eWAT, iWAT, and Gast. M. Kaplan–Meier analysis of mouse survival (n = 10 per group), with comparisons performed using the log-rank test. Statistical analyses were performed using the one-way ANOVA (A, D, F, G, H–L) or the log-rank test (O). Data are shown as the mean ± SEM