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Fig. 7 | Journal of Hematology & Oncology

Fig. 7

From: LCN2 secreted by tissue-infiltrating neutrophils induces the ferroptosis and wasting of adipose and muscle tissues in lung cancer cachexia

Fig. 7

Chemical inhibition of ferroptosis reduces tissue wasting in lung cancer cachexia. A–D. Lung cancer cachexia model mice were treated with the ferroptosis inhibitor, liproxtatin-1 (10 mg per kg body weight, delivered by intraperitoneal injection daily between days 1 and 20), and the tissues were harvested on day 21. A. Representative H&E staining of the iWAT, eWAT, and Gast. Scale bars, 100 μm. B. Fe2+ concentration in the iWAT, eWAT, and Gast. n = 5–6 per group. C. Flow cytometry analyses of the relative lipid ROS levels in the adipocytes of the eWAT and iWAT. A PE channel was used to detect non-oxidized lipids and a FITC channel was used to detect oxidized lipids. The FITC to PE MFI ratio was calculated as the relative lipid ROS value. n = 5–6 per group. D. Chemiluminescence analysis of the MDA concentration in the iWAT, eWAT, and Gast. n = 6 per group. E–L. Lung cancer cachexia model mice were treated with the ferroptosis inhibitor, DFO (15 mg per kg body weight, delivered by intraperitoneal injection every 3 days between days 4 and 19), and the tissues were harvested on day 21. (E, F) Concentrations of (E) Fe2+ and (F) MDA in the eWAT. n = 5 per group. (G, H) Flow cytometry analyses of (G) relative lipid ROS and (H) the percentage of 7-AAD+ adipocytes in the eWAT. n = 5 per group. I. Representative images (left) and body weights (right) of the different groups of mice. n = 5 per group. J. Representative images (left) and weights (right) of the eWAT and iWAT. n = 5 per group. K. Representative H&E staining of the iWAT. Scale bars, 100 μm. L. Kaplan–Meier analysis of mouse survival (n = 10 per group), with comparisons performed using the log-rank test. Statistical analyses were performed using the one-way ANOVA (B–I, J). Data are shown as the mean ± SEM

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