Fig. 7From: LCN2 secreted by tissue-infiltrating neutrophils induces the ferroptosis and wasting of adipose and muscle tissues in lung cancer cachexiaChemical inhibition of ferroptosis reduces tissue wasting in lung cancer cachexia. A–D. Lung cancer cachexia model mice were treated with the ferroptosis inhibitor, liproxtatin-1 (10 mg per kg body weight, delivered by intraperitoneal injection daily between days 1 and 20), and the tissues were harvested on day 21. A. Representative H&E staining of the iWAT, eWAT, and Gast. Scale bars, 100 μm. B. Fe2+ concentration in the iWAT, eWAT, and Gast. n = 5–6 per group. C. Flow cytometry analyses of the relative lipid ROS levels in the adipocytes of the eWAT and iWAT. A PE channel was used to detect non-oxidized lipids and a FITC channel was used to detect oxidized lipids. The FITC to PE MFI ratio was calculated as the relative lipid ROS value. n = 5–6 per group. D. Chemiluminescence analysis of the MDA concentration in the iWAT, eWAT, and Gast. n = 6 per group. E–L. Lung cancer cachexia model mice were treated with the ferroptosis inhibitor, DFO (15 mg per kg body weight, delivered by intraperitoneal injection every 3 days between days 4 and 19), and the tissues were harvested on day 21. (E, F) Concentrations of (E) Fe2+ and (F) MDA in the eWAT. n = 5 per group. (G, H) Flow cytometry analyses of (G) relative lipid ROS and (H) the percentage of 7-AAD+ adipocytes in the eWAT. n = 5 per group. I. Representative images (left) and body weights (right) of the different groups of mice. n = 5 per group. J. Representative images (left) and weights (right) of the eWAT and iWAT. n = 5 per group. K. Representative H&E staining of the iWAT. Scale bars, 100 μm. L. Kaplan–Meier analysis of mouse survival (n = 10 per group), with comparisons performed using the log-rank test. Statistical analyses were performed using the one-way ANOVA (B–I, J). Data are shown as the mean ± SEMBack to article page