XPO1 expression worsens the prognosis of unfavorable DLBCL that can be effectively targeted by selinexor in the absence of mutant p53

The XPO1 inhibitor selinexor was recently approved in relapsed/refractory DLBCL patients but only demonstrated modest anti-DLBCL efficacy, prompting us to investigate the prognostic effect of XPO1 in DLBCL patients and the rational combination therapies in high-risk DLBCL. High XPO1 expression (XPO1high) showed significant adverse prognostic impact in 544 studied DLBCL patients, especially in those with BCL2 overexpression. Therapeutic study in 30 DLBCL cell lines with various molecular and genetic background found robust cytotoxicity of selinexor, especially in cells with BCL2-rearranged (BCL2-R+) DLBCL or high-grade B-cell lymphoma with MYC/BCL2 double-hit (HGBCL-DH). However, expression of mutant (Mut) p53 significantly reduced the cytotoxicity of selinexor in overall cell lines and the BCL2-R and HGBCL-DH subsets, consistent with the favorable impact of XPO1high observed in Mut-p53-expressing patients. The therapeutic effect of selinexor in HGBCL-DH cells was significantly enhanced when combined with a BET inhibitor INCB057643, overcoming the drug resistance in Mut-p53-expressing cells. Collectively, these data suggest that XPO1 worsens the survival of DLBCL patients with unfavorable prognostic factors such as BCL2 overexpression and double-hit, in line with the higher efficacy of selinexor demonstrated in BCL2-R+ DLBCL and HGBCL-DH cell lines. Expression of Mut-p53 confers resistance to selinexor treatment, which can be overcome by combined INCB057643 treatment in HGBCL-DH cells. This study provides insight into the XPO1 significance and selinexor efficacy in DLBCL, important for developing combination therapy for relapsed/refractory DLBCL and HGBCL-DH.

inhibitor selinexor has received FDA approval recently to treat refractory/relapsed (R/R) diffuse large B-cell lymphoma (DLBCL) after at least 2 lines of systemic therapy, showing an overall response rate of 28% in the SADAL trial [3]. However, it remains largely unknown whether and how XPO1 interplays with other adverse predictors in DLBCL, how to predict selinexor effectiveness, and what combination therapy is optimal in R/R DLBCL patients. Here, we evaluated the prognostic significance of XPO1 expression in 544 well-characterized DLBCL cases, and investigated the therapeutic effect of selinexor in 30 DLBCL cell lines with variable genetic background.
Patients and Methods for this study are detailed in Additional file 1. XPO1 expression was observed in 217 of 544 (40%) DLBCL patients with a mean level of 24%.
Whether XPO1 high interacts with other adverse prognostic factors and whether XPO1 is a potential   (Fig. 1b,c), which is known as double-expressor lymphoma with unfavorable prognosis [6]. Trends of adverse impact were also observed on PFS in MYC-rearranged (R + ) patients (P = 0.097; Additional file 1: Figure S1C) and OS in patients with dual MYC-R + BCL2-R + (Fig. 1c) with dismal prognosis, defined as high-grade B-cell lymphoma with MYC/BCL2 double-hit (HGBCL-DH) [7]. In patients with TP53 mutation (Mut-TP53) [8], XPO1 high showed opposite prognostic effects in patients with and without Mut-p53 protein overexpression [9], suggesting the nuclear export may attenuate the oncogenic gain-of-function of Mut-p53. In contrast to the negative impact of XPO1 high in Mut-TP53/p53-negative patients (Fig. 1d) and in TP53wild type (Wt-TP53) patients (Additional file 1: Figure  S1D), a favorable effect was associated with XPO1 high in Mut-TP53/p53-positive patients, which was significant in the BCL2 low subset (Fig. 1e). Gene expression profiling [4] analysis identified a distinct gene expression signature for XPO1 high in patients with Mut-TP53 and MYC high (Fig. 1f ), including upregulation of SIRPA, which encodes SIRPα, a receptor for CD47 transmitting "do not eat me" signal in phagocytosis, and downregulation of several genes related to DNA repair, metabolism, splicing, or biosynthesis (Additional file 1: Table S2). Next, selinexor was assessed in 30 DLBCL cell lines, which resulted in significantly reduced cell viability with varying IC50 values (Fig. 2a). ABC-DLBCL and GCB-DLBCL cells were similarly vulnerable to selinexor   Figure S1E), consistent with results in the SADAL clinical trial [3]. Biomarkers significantly associated with higher sensitivity (lower IC50) to selinexor cytotoxicity included BCL2-R and HGBCL-DH (Fig. 2b) but not MYC-R. In contrast, presence of Mut-TP53/p53 + significantly reduced the anti-lymphoma efficacy of selinexor, especially in HGBCL-DH cells (Fig. 2c; Additional file 1: Figure S1F).
Limited efficacy of selinexor in HGBCL with Mut-TP53/p53 + calls for combination strategy. Previous studies showed the synergy between selinexor and venetoclax in DLBCL and double-hit lymphoma [10,11]. However, in the SADAL trial [3], patients with MYC high (but not BCL2 high ) expression had a lower overall response rate than those without. MYC expression can be inhibited by targeting the bromodomain and extra-terminal domain (BET) proteins [12]. We therefore combined selinexor with a novel BET inhibitor INCB057643. Synergistic effect was observed in DLBCL/HGBCL cells, especially in HGBCL-DH cells with Mut-TP53/p53 + (Fig. 2d), supporting INCB057643/selinexor combination as a therapeutic option for HGBCL-DH patients.
In summary, this study demonstrates that XPO1 high is a valuable biomarker in DLBCL with unfavorable prognostic factors, predictive of significantly poorer outcomes in ABC-DLBCL, BCL2 high DLBCL, and double-expressor lymphoma but not Mut-p53-expressing DLBCL. Targeting XPO1 with selinexor is similarly effective in GCB-DLBCL and ABC-DLBCL cells, and remarkably effective in BCL2-R + DLBCL and HGBCL cells without Mut-TP53/p53-positivity. In DLBCL/HGBCL cells, Mut-TP53/p53-positive expression predicts resistance to selinexor. INCB057643 synergizes with selinexor in HGBCL-DH cells, overcoming resistance in Mut-TP53/ p53-positive HGBCL-DH. These findings warrant future investigation on the role of XPO1, selinexor, and combined BET inhibition in R/R DLBCL and HGBCL-DH.
Additional file 1. Table S1: Clinicopathologic and molecular characteristics of DLBCL patients with high or low XPO1 expression. Table S2: Significantly differentially expressed genes between XPO1 high and XPO1 low DLBCL patients with concurrent TP53 mutation and high MYC expression.