Figure 6
From: Effect of Ras Inhibition in Hematopoiesis and BCR/ABL Leukemogenesis
Structure of retroviral vectors used to transduce GFP, BCR/ABL-GFP and BCR/ABL-GFP + N17 H-Ras and their expression in NIH 3T3 cells. (A) LTR: long terminal repeat. Enzyme abbreviations: Cl, Cla I; RI, EcoR I; N, Not I; S, Sal I. (B) NIH 3T3 cells were infected with titer-matched MSCV-IRES-GFP (lane 1), MSCV-BCR/ABL-GFP-IRES-Neo (lane 2), and MSCV- BCR/ABL-GFP-IRES-N17 H-Ras (lane 3) retroviruses. The corresponding cell lysates were subjected to SDS-PAGE and analyzed by Western blot, probed with anti-Abl monoclonal antibody AB-3, anti-Ras monoclonal antibody and anti-dynamin (as a loading control).