Figure 5

SET-NUP214 protein expression. Western blot analysis with Ab raised against the N-terminal region of SET and against the C-terminal region of NUP214. Cell lines LOUCY and MEGAL expressed the 140 kDa SET-NUP214 fusion protein and a 240 kDa protein marked with an asterisk, detected by both antibodies. No alternative splice forms were detected that would explain two SET-NUP214 size variants.