Figure 1
Structure of small molecule inhibitor TW-37. Growth inhibition effect of TW-37 on 4 NHL cell lines and fresh cells obtained from 8 patient samples. Data represent IC50 at 72 hr from TW-37 exposure using trypan blue exclusion method. A) WSU-pre-ALL cell line (A1); WSU-DLCL2 cell line (A2); WSU-FSCCL cell line (A3) and WSU-WM cell line (A4). Cell lines were seeded in 24-well culture clusters at a density of 2 × 105 viable cells/ml per well. Triplicate wells were treated with 0.0–750 nM TW-37 and incubated for up to 72 hr. B) Fresh patient derived cells were seeded in 24-well culture clusters at a density of 5 × 105 viable cells/ml per well. Triplicate wells were treated with 0.00–750 nM TW-37 and incubated for 72 hr. Cytotoxic effect of TW-37 on primary NHL cells is at 72 hr. C) Cytotoxic effect of TW-37 on normal peripheral blood lymphocytes was assayed by seeding 4 × 105 viable cell/ml and treated with 0.00–800 nM of TW-37 for up to 72 hr.