Basic Mechanisms of Arsenic Trioxide (ATO)-Induced Apoptosis in Human Leukemia (HL-60) Cells

Table 1 Summary data of annexin V assay obtained from the flow cytometry analysis.

ATO Concentrations	Annexin-V Negative Cells or Viable Cells	Annexin-V Positive Cells or Apoptotic Cells
	(Mean ± SD)%	(Mean ± SD)%
0 μg/mL	99.0 ± 0.0	1.0 ± 0.0
2 μg/mL	88.5 ± .07	11.5 ± 0.7
4 μg/mL	80.4 ± 5.7*	19.6 ± 5.7*
6 μg/mL	64.2 ± 5.3*	35.8 ± 5.3*
8 μg/mL	82.4 ± 0.5*	17.6 ± 0.5*

HL-60 promyelocytic leukemia cells were cultured in the absence or presence of ATO for 24 h as indicated in the Materials and Methods. Values are shown as means ± SDs of 3 replicates per experiment. *Significantly different at p < 0.05 to the control group.

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