Figure 6
Effect of 5-MF and 2'-MF on DR4 and DR5 expression in MOLT-4 cells. The cells were treated with each MF derivative (at IC20 level) for various times and then treated with TRAIL (IC20) for 24 h. The whole-cell extracts were prepared and analyzed by Western blotting using anti-DR4 and anti-DR5 antibodies. DR4 (A) and DR5 (B) expression of MOLT-4 cells treated with 5-MF are shown. The cells were also treated with 2'-MF for various times of incubation and TRAIL for 24 h, DR4 (C) and DR5 (D) expression are shown. The intensity of bands was quantified by densitometry. *, p < 0.05, compared with control.