Table 1 Primer sequences used in this study
Real-time PCR analysis | |||
|---|---|---|---|
Target gene | Forward (5′ → 3′) | Reverse (5′ → 3′) | Probe (5′ → 3′) |
MCPyV ST | GCAAAAAAACTGTCTGACGTGG | CCACCAGTCAAAACTTTCCCA | FAM-TATCAGTGCTTTATTCTTTGGTTTGGATTTCCTCCT- TAMRA |
HPyV9 VP1 | TGCTGTTGATATTGTTGGAATTCA | AACAACCCGTTTCCTTAGAGTTACA | FAM-CTGGAGAGGCCTACCT-NFQ-MGB |
Human RNase P | AGATTTGGACCTGCGAGCG | GAGCGGCTGTCTCCACAAGT | FAM-TTCTGACCTGAAGGCTCTGCGCG-TAMRA |
PCR analysis | |||
Target gene (positions) | Forward (5′ → 3′) | Reverse (5′ → 3′) | |
MCPyV LT (1867–2221) | AGCCCCCTTACAAATTACTGCAAG* | AGCATTTCTGTCCTGGTCATTTC* | |
MCPyV ST-LT (183–828) | GCATATAGACAAGATGGATTTA† | ATAACCTTTCTTTGATATTTTGC† | |
MCPyV ST-LT (571–1157) | TTGTCTCGCCAGCATTGTAG† | GGATCCAGAGGATGAGGTGGGTTC† | |