Figure 1From: TFPIα and TFPIβ are expressed at the surface of breast cancer cells and inhibit TF-FVIIa activityTFPI expression in different breast cancer cell lines and normal cells. Cells were seeded in 6-wells trays to reach ~80% confluence in three days before media were collected and cells lysed for RNA isolation. (A) Relative TFPIα and TFPIβ mRNA expression measured using qRT-PCR. ΔΔCt values were calculated using 18s rRNA as an endogenous control and the TFPI negative, non-human CHO cells as a negative control. (B) Secreted TFPIα protein measured in cell media and normalized to total protein amounts. Mean values + SD (n = 3) are presented.Back to article page