Figure 4

The PI3K/mTOR pathway contributes to the expansion of definitive myelopoiesis in pten −/− embryos. (A-L) Whole-mount in situ hybridization analyses of lyz- positive myeloid cells in embryos treated with increasing doses of LY294002 (LY) or rapamycin (Ra) at 90 hpf. Untreated and mock-treated pten−/− embryos (B, C and H, I) showing expansive myelopoiesis compared to the controls (A and G), as mentioned above. The pten−/− embryos treated with stepwise increases in LY294002 or rapamycin showed an obvious rescue effect, as indicated by the decrease in myeloid cells (D-F and I-L). The red arrowheads indicate the main positions of the myeloid cells. (M and N) Quantification of myeloid cells in each embryo (a-l). The data shown are the means ± SEM of at least 30 embryos, *p < 0.05, **p < 0.01, ***p < 0.001 versus the corresponding controls. Scale bar: 100 um.