Figure 2
Effect of IFN-γ exposure on constitutive and immunoproteasome subunits mRNA and protein levels in bortezomib-resistant and bortezomib-sensitive 8226 (MM), THP1 (AML) and CEM (ALL) cells. (A) Expression levels of β5, β1, β2, β5i, β1i and β2i mRNA were monitored after 6-72 hrs exposure of 8226/BTZ, THP1/BTZ, CEM/BTZ and (B) their parental counter parts to 100 U/ml IFN-γ. Results are presented relative to untreated controls conditions and depicted as mean (± SD) of 3 individual experiments for bortezomib-resistant cells and mean of two experiments performed in duplicate for parental cells. (C) Western blot analysis of β1i, β1, β5i and β5 protein expression after 6-72 hrs exposure of 8226/BTZ, THP1/BTZ, CEM/BTZ and (D) their parental counter parts to 100 U/ml IFN-γ. One representative example of 3 experiments is shown.