Fig. 7From: Long non-coding RNA ANRIL is upregulated in hepatocellular carcinoma and regulates cell proliferation by epigenetic silencing of KLF2ANRIL negatively regulates expression of KLF2 by rescue assays. a, b Colony-forming assays were used to determine the cell viability for HepG2 cells transfected with si-NC and si-ANRIL and co-transfected with siANRIL and si-KLF2. Values represent the mean ± s.d. from three independent experiments. c MTT assays were used to determine the cell viability for HepG2 cells transfected with si-NC and si-ANRIL and co-transfected with siANRIL and si-KLF2. Values represent the mean ± s.d. from three independent experiments. d, e The levels of KLF2 protein levels were determined by Western blotting when HepG2 cells transfected with si-NC and si-ANRIL and co-transfected with siANRIL and si-KLF2. *P < 0.05, **P < 0.01Back to article page