Lung tumor exosomes induce a pro-inflammatory phenotype in mesenchymal stem cells via NFκB-TLR signaling pathway

Table 1 Gene lists of human toll-like receptor signaling pathway and data for 3D profile

3D profile gene data	A	B	C	D	E	F	G	H
1	BTK	ECSIT	IKBKB	IRF3	MYD88	RELA	TLR3	ACTB
1	0.64	0.86	0.88	0.84	1.63	1.17	1.39	1.14
2	CASP8	EIF2AK2	IL10	JUN	NFKB1	RIPK2	TLR4	B2M
2	0.86	1.16	0.41	0.75	1.77	1.06	0.74	1.16
3	CCL2	ELK1	IL12A	LTA	NFKB2	SARM1	TLR5	GAPDH
3	13.80	0.85	1.35	0.91	0.41	0.81	1.71	0.87
4	CD14	FADD	IL1A	LY86	NFKBIA	SIGIRR	TLR6	HPRT1
4	1.00	0.91	0.60	0.82	3.01	1.19	0.68	1.09
5	CD180	FOS	IL1B	LY96	NFKBIL1	TAB1	TLR7	RPLPO
5	2.57	1.18	12.43	1.02	0.27	0.83	7.73	0.79
6	CD80	HMGB1	IL2	MAP2K3	NFRKB	TBK1	TLR8
6	2.57	0.65	0.82	1.10	0.86	0.71	0.55
7	CD86	HRAS	IL6	MAP2K4	NR2C2	TICAM1	TLR9
7	0.82	0.27	21.55	0.95	0.85	1.58	0.71
8	CHUK	HSP1A1	IL8	MAP3K1	PELT1	TICAM2	TNF
8	1.01	1.12	19.37	0.89	1.23	0.75	2.50
9	CLEC4E	HSPD1	IRAK1	MAP3K7	PPARA	TIRAP	TNFRSF1A
9	0.82	0.82	0.91	0.96	0.79	0.88	1.07
10	CSF2	IFNA1	IRAK2	MAP4K4	PRKRA	TLR1	TOLLIP
10	9.57	0.89	1.79	0.87	1.03	0.48	1.58
11	CSF3	IFNB1	IRAK4	MAPK8	PTGS2	TLR10	TRAF6
11	43.95	1.42	1.58	0.86	4.75	0.30	0.91
12	CXCL10	IFNG	IRF1	MAPK8IP3	REL	TLR2	UBE2N
12	217.01	0.82	2.03	0.74	1.19	5.64	0.94

MSCs were treated with or without 200 μg/ml A549 cell-derived exosomes for 24 h. Total RNA were analyzed using human toll-like receptor signaling pathway array. Fold change of RNA expression was used for 3D profile (200–24 h/0–24 h)

ISSN: 1756-8722