Table 2 Primers for RT-PCR
Gene | Forward primer and reverse primer (5'–3') |
|---|---|
ALP | CCACGTCTTCACATTTGGTG; AGACTGCGCCTGGTAGTTGT |
RUNX2 | TGTCATGGCGGGTAACGAT; AAGACGGTTATGGTCAAGGTGAA |
OCN | GGCGCTACCTGTATCAATGG; GTGGTCAGCCAACTCGTCA |
PPAR γ | CCTATTGACCCAGAAAGCGATT; CATTACGGAGAGATCCACGGA |
LPL | ACAAGAGAGAACCAGACTCCAA; AGGGTAGTTAAACTCCTCCTCC |
C/EBPβ | CTTCAGCCCGTACCTGGAG; GGAGAGGAAGTCGTGGTGC |
IL-6 | ACTCACCTCTTCAGAACGAATTG;CCATCTTTGGAAGGTTCAGGTTG |
IL-8 | ACTCCAAACCTTTCCACCCC; TTCTCAGCCCTCTTCAAAAACTTC |
MCP-1 | CAGCCAGATGCAATCAATGCC; TGGAATCCTGAACCCACTTCT |
IL-1β | AGCTACGAATCTCCGACCAC; CGTTATCCCATGTGTCGAAGAA |
TNF-α | CCTCTCTCTAATCAGCCCTCTG; GAGGACCTGGGAGTAGATGAG |
IFN-α | GCCTCGCCCTTTGCTTTACT; CTGTGGGTCTCAGGGAGATCA |
IFN-β | GCTTGGATTCCTACAAAGAAGCA;ATAGATGGTCAATGCGGCGTC |
IFN-γ | TCGGTAACTGACTTGAATGTCCA; TCGCTTCCCTGTTTTAGCTGC |
ACTA2 | CGATGCTCCCAGGGCTGTTT; TTCGTCACCCACGTAGCTGTCTTT |
TLR1 | CCACGTTCCTAAAGACCTATCCC; CCAAGTGCTTGAGGTTCACAG |
TLR2 | ATCCTCCAATCAGGCTTCTCT; GGACAGGTCAAGGCTTTTTACA |
TLR3 | TTGCCTTGTATCTACTTTTGGG; TCAACACTGTTATGTTTGTGGGT |
TLR4 | AGACCTGTCCCTGAACCCTAT; CGATGGACTTCTAAACCAGCCA |
TLR5 | TCCCTGAACTCACGAGTCTTT; GGTTGTCAAGTCCGTAAAATGC |
TLR6 | TGAATGCAAAAACCCTTCACC; CCAAGTCGTTTCTATGTGGTTGA |
TLR7 | CACATACCAGACATCTCCCC; CCCAGTGGAATAGGTACACAGTT |
TLR8 | ATGTTCCTTCAGTCGTCAATGC; TTGCTGCACTCTGCAATAACT |
TLR9 | CTGCCACATGACCATCGAG; GGACAGGGATATGAGGGATTTGG |
TLR10 | GGTTCTTTTGCGTGATGGAATC; GTCGTCCCAGAGTAAATCAAC |
GAPDH | GGTCACCAGGGCTGCTTTTA; GGATCTCGCTCCTGGAAGATG |