Fig. 4

The induction of HOTAIRM1 is dependent on PU.1 during granulocytic differentiation. The expression of PU.1, HOTAIRM1, ITGAM, and HNRNPH1 after non-targeting or PU.1 siRNA transfection in NB4 cells, with ATRA treatment for 24 h. An average of two control genes (GAPDH and ACTB) was used for normalization. The data represent the mean of three replicates ± SD. *p < 0.05; **p < 0.01; ***p < 0.001