Fig. 4

Anti-tumor activity of FRα CAR T cells in vitro and in vivo. a FRα CAR T cells lysed FRα+ MDA-231 cells but exhibited decreased lysis of the FRα-C30 cells at the indicated effector/target (E/T) ratio for ~20 h. Untransduced (UNT) T cells served as our negative control. b NSG mice bearing established subcutaneous (s.c.) tumor were treated with intravenous (i.v.) injections of 1 × 10⁷ CAR+ T cells on days 40 and 46 post tumor inoculation. Tumor growth was assessed by caliper measurement [V = 1/2(length × width²)]. c Peripheral blood was collected 3 weeks after the first T cell infusion and quantified for the absolute number of human CD4+ and CD8+ T cells/μL of blood. Mean cell count ± SEM is shown with n = 5 for all groups