Fig. 3

MAGL promotes the progression of HCC by enhancing EMT. a The cellular morphology of HCC cell lines (HepG2-vector, HepG2-MAGL, HCCLM3-vector, and HCCLM3-shMAGL). b Western blot assay revealed the expression of epithelial and mesenchymal markers, as well as the transcription factors, in HCC cells with different MAGL expression (HepG2-vector, HepG2-MAGL, HCCLM3-vector, and HCCLM3-shMAGL). c–g qRT-PCR analysis of E-cadherin, N-cadherin, Vimentin, Snail, Twist levels in the indicated cell lines. h Representative immunostaining images of EMT markers using serial sections of tumor samples. Scale bar, 50 μm. Data represent the mean + SD. *P < 0.05, **P < 0.01, and ***P < 0.001