Fig. 1

DC-SIGNR regulates colon cancer cell adhesion. a The DC-SIGNR protein was detected by Western Blot. b LoVo, LS174T, and HCT-116 cells were incubated with the recombinant DC-SIGNR protein on ice for 3 h. The adhesion complexes were detected by an antibody against DC-SIGNR and a FITC-conjugated goat anti-mouse IgG secondary antibody and analysed by flow cytometry. The adhesive ratio of the three colon cancer cell lines was 72.30% for the LoVo cells, 82.84% for the LS174T cells and 70.47% for the HCT-116 cells. c, d LoVo cells were treated with different concentrations of the DC-SIGNR protein (1, 2 and 5 μg/ml). For the inhibition assay, the DC-SIGNR (5 μg/ml) protein was pre-incubated with EDTA, galactose, D-mannose, N-acetylglucosamine, or L-fucose. The binding of DC-SIGNR to LoVo cells was dose-dependent, and the interaction could be inhibited by EDTA and monosaccharides. ***P < 0.001. The error bars in all graphs represent SD, and each experiment was repeated three times