Fig. 1From: Pterostilbene prevents AKT-ERK axis-mediated polymerization of surface fibronectin on suspended lung cancer cells independently of apoptosis and suppresses metastasisEndogenous FN expression is required for polyFN assembly on suspended LLC cells, which promotes lung metastasis. a The IB was probed with anti-FN pAb (upper and middle panel) or anti-GAPDH (lower) as input control for the whole cell lysates of LLC cells alone (control) or LLC cells expressing Scr shRNA (Scr) or FN shRNAs (shFN#1 and shFN#2) that were resolved in SDS-PAGE under reducing conditions. The polyFN means polymeric FN form (upper panel) and monoFN monomeric FN form (middle panel). GT means well-top of stacking gel. b The quantifications of expression levels of polyFN (% polyFN; left panel) and monoFN (% monoFN) by ImageJ software as normalized with expression levels of GAPDH. c Tumor nodule numbers on mouse lung surfaces were counted, and d mouse lungs were taken from mice that intravenously received LLC cells as described in (a) upon mouse sacrifices. e H&E staining for tumor nodules in mouse lungs in (d). Two representative lungs of each group were demonstrated. Note: arrow heads depict tumor nodules in the lungsBack to article page