Fig. 4

ELF2B overexpression induces apoptosis in vitro. Annexin V-labelling of HeLa (a) and HFF cells (b) overexpressing ELF proteins; cells recovered for 18 h after UV exposure were included as a positive control. c Annexin V assay of ELF2B expressing cells incubated ± Dox for 3 d. d Caspase 3/7 activation assay performed in HeLa cells normalised to non-transduced HeLa control cells; statistical analysis is performed compared to GFP control. Data represents the mean ± SEM of three experiments with statistical analysis performed using Student’s t test (ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001)