Fig. 5

miR-455-5p modulates the expression of genes involved in interferon response. A Volcano plot showing differentially expressed genes found by microarray analysis. Left panel: pN2 vs. pN0 (MED samples). Central panel: NCI-H1993 CTRL vs NCI-H1993 OE cells (N = 2). Right panel: CDDP-R CTRL (N = 2) vs CDDP-R OE cells (N = 2). Gray dot, unchanged genes; Blue dot, downregulated genes (p value < 0.05; FC < -1.5); Red dot, upregulated genes (p value < 0.05; FC > 1.5). P value was calculated using the Limma moderated t test. B GSEA using miR-455-5p-predicted target genes in pN2 vs. pN0 (MED samples), H1993 CTRL vs H1993 OE or CDDP-R CTRL vs CDDP-R OE. NES, normalized enrichment score; FDR, false-discovery rate. (C) Circular plot showing GSEA results using the ‘Hallmark gene sets’ collection in pN2 vs pN0 (MED samples), H1993 CTRL vs H1993 OE and CDDP-R CTRL vs CDDP-R OE. In red, common enriched gene signatures having the same trend of regulation in all experimental conditions. D and E GSEA of (D) IFN-α and (E) IFN-γ response gene sets in pN2 vs pN0 (MED samples), H1993 CTRL vs H1993 OE and CDDP-R CTRL vs CDDP-R OE. F Venn diagram representing the overlap of genes between IFN-α/IFN-γ response gene sets and miR-455-5p target genes