Fig. 1

Molecular design and preclinical evaluation of ET019003. A Schematic structure of ET019003 compared with natural γ/δ TCR and ET019L1-CAR. L, light; H, heavy; scFv, single-chain fragment variable. B Cytotoxicity of ET019003 and ET019L1-CAR-T cells on CD19⁺ Raji and Nalm-6 cells (n = 5). C Production of interleukin (IL)-2, granulocyte macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ) in coculture of ET019L1-CAR-T and ET019003 cells with Raji and Nalm-6 cells (n = 5). D Luciferase live imaging of Raji xenograft mice on different timepoint after receiving mock T cells, ET019L1-CAR-T cells, and ET019003 cells. E Kaplan–Meier survival plot of Raji xenograft mice. F Serum IL-2, IL-6, GM-CSF, TNF-α, and IFN-γ in Raji xenograft mice on day 7 after infusion of ET019L1-CAR-T and ET019003 cells (n = 6). The unpaired t test was used in Fig. A–C and F, and the log-rank test was used in Fig. E. Ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001