Fig. 6

Killing of MM cells in patient-derived bone marrow samples. Killing of patient-derived bone marrow MM samples was assessed by FACS-based cytotoxicity experiments in which TCR Td T cells were co-cultured with patient BM samples in an E/T ratio 3:1. MM cell survival was analyzed after overnight co-culture. A Example of survival of an HLA-A1^pos/A24^pos patient sample after co-culture with CD8 T cells transduced with a CMV (negative control), Jchain HLA-A1 or Jchain HLA-A24 restricted TCR. In red MM cells are displayed, highlighted by black boxes for clarity, MM cells were gated on: live cells → single cells → CD3-negative cells to exclude co-cultured T cells → CD45 negative-intermediate, CD19 negative → CD56 positive, CD38 positive. MM cells (in red) were backgated on total CD3-negative cells (in blue). B MM.J1-MMJ.10 codes in graph titles represent different MM patients, additionally expression of target HLA molecules and JCHAIN expression as a fold increase relative to housekeeping genes is displayed. JCHAIN expression was measured by qPCR on sorted MM cells. Numbers of surviving MM cells acquired per 2500 Flow-Count Fluorospheres are displayed. Jchain HLA-A1 and HLA-A24 TCR Td T CD8 T cells co-cultured with MM patient samples from different individuals expressing HLA-A1, A24 or both (top row). Jchain HLA-A3 and HLA-A11 TCR Td T CD8 T cells co-cultured with MM patient samples from different individuals expressing HLA-A3 or A11 (bottom row). Technical triplicates are shown. Data representative of two independent experiments