| Sequence | Jchain aa | Eluted from | HLA-Ic | %Rank_ELd | WB/SBe | HLA binding |
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Positiona | MM cell lineb | Confirmedf |
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YTA-A1 | YTAVVPLVY | 132–140 | UM9 | A1 | 0.02 | SB | Yes |
TAV-A1 | TAVVPLVY | 133–140 | UM9 | A1 | 1.30 | WB | Yes |
VLA-A21 | VLAVFIKAVHV | 10–20 | U266 | A2 | 3.22 |  < WB | Yes |
VLA-A22 | VLAVFIKAV | 10–18 | UM9 and U266 | A2 | 0.23 | SB | Yes |
YTA-A2 | YTAVVPLV | 132–139 | UM9 | A2 | 4.17 |  < WB | Yes |
ISD-A3 | ISDPTSPLRTR | 72–82 | U266 | A3 | 3.16 |  < WB | Yes |
RII-A11 | RIIVPLNNR | 61–69 | UM9 | A11 | 0.24 | SB | Yes |
ISD-A11 | ISDPTSPLRTR | 72–82 | UM9 | A11 | 1.90 | WB | Yes |
CYT-A24 | CYTAVVPLV | 131–139 | U266g | A24 | 0.96 | WB | Yes |
- aAmino acid (aa) position of identified peptides within the Jchain protein according to UniProt
- bUM9 cells (HLA-A1, -A11, -B7, -B35, -C3 and -C7 positive) were HLA-A2-transduced and U266 cells (HLA-A2, -A3, -B7, -B40, -C3, and -C7 positive) were HLA-A24-transduced
- cMost likely HLA-I origin of peptides based on HLA typing of MM cells from which peptides were eluted and peptide-binding motifs of these HLA alleles according to NetMHC4.0
- dRank of the predicted binding score for the eluted peptide to the respective HLA molecule compared to a set of random natural peptides
- ePeptides were annotated as weak or strong binders using the netMHC4.1 default setting of 0.5% rank for strong binders (SB) and 2% rank for weak binders (WB). Peptides with %Rank > 2.0 were annotated as < WB
- fPeptide binding to the respective HLA allele was investigated by peptide-HLA monomer refolding. Yes: peptide-HLA monomers were successfully refolded and remained stable. No: peptide-HLA monomers could not stably be refolded
- gEpitope identified in HLA-peptide elution experiment using anti-HLA-A1/A24 antibody but not in elution using pan HLA-I antibody W6-32