Fig. 3
From: Tumor cell integrin β4 and tumor stroma E-/P-selectin cooperatively regulate tumor growth in vivo
Combined depletion of ITGB4 and E-/P-selectin cooperatively reduces human ovarian cancer xenograft tumor formation. shRNA-mediated knockdown of ITGB4 in SKOV3 cells, survival of mice after i. p. injection of shControl vs. shITGB4 cells into E-/P-selectin wildtype vs. knockout SCID mice (endpoint: palpable abdominal tumor masses or ascites) and representative anti-ITGB4 immunostaining images from i. p. SKOV3 xenograft tumors (A). Intraperitoneal carcinosis score (ICS) determined as illustrated and spontaneous pulmonary metastatic cell load determined by Alu-PCR on day 57 after i. p. injection of SKOV3 shControl vs. shITGB4 cells into E-/P-selectin wildtype vs. knockout SCID mice (B). Representative anti-ITGB4 immunostaining images of spontaneous SKOV3 lung metastases (arrowhead indicates an ITGB4− lung metastasis) (B). All statistical comparisons indicated by asterisks were calculated vs. shControl/ WT. ***p < 0.001