Fig. 5

Depletion of TI-Neu cells prevents tissue ferroptosis and wasting in lung cancer cachexia. Mice were inoculated subcutaneously with LLC cells to induce cachexia and were treated with 100 μg anti-Ly6G antibody (or IgG control) three times per week between days 1 and 20; the tissues were harvested on day 21. A. Representative images (left) and body weights (right) of the lung cancer cachexia model mice. n = 5 per group. B. Representative images (left) and weights (right) of the eWAT. n = 5 per group. C. Representative images (left) and weights (right) of the iWAT. n = 5 per group. D. Representative H&E staining of the iWAT, eWAT, and Gast. Scale bars, 100 μm. E. qPCR analysis of Lcn2 mRNA levels in the eWAT, iWAT, and Gast. n = 5 per group. F. Serum LCN2 concentrations. n = 5 per group. G. MDA concentrations in the eWAT. n = 5–6 per group. H. MDA concentrations in the iWAT and Gast. n = 6 per group. Data are shown as the mean ± SEM. Statistical analyses were performed using the one-way ANOVA (A–C, E–H)