Fig. 2

The hnCD16 fusion receptors induce alterations in the transcriptome of NK cells. A Principal component analysis of gene expression analyses of the indicated NK cell populations. B Heatmap showing unsupervised clustering analysis based on immune function-related genes (n = 3). C Unsupervised hierarchical clustering analysis based on genes associated with NK cell-mediated cytotoxic pathways. D Upset plot showing the numbers and overlaps between differentially expressed genes (DEGs) in various inter-group comparison sets. Each row indicates a DEG set, while each column indicates a combination of DEG sets, as indicated by the linked dots. The bar plot on the left side represents the number of genes of each DEG set and that on the upper side represents the size of overlap between different DEG sets. E–G Top GO terms of biological processes enriched in DEGs between hnCD16 NK cells versus Control NK cells (E), FR1 NK cells versus Control NK cells (F), and FR1 NK cells versus hnCD16 NK cells (G)