Fig. 5

Phenotypic characterization of iPSC-derived NK cells expressing hnCD16FR and anti-tumor function via ADCC. A Schematic illustration of the steps leading from human iPSCs to hnCD16FR-iNK cells. B Representative images showing CTRL(eGFP)-iPSCs and hnCD16FR-expressing iPSCs (up) and CD16 expression (down) by flow cytometry. C Flow cytometry analysis of NK cell surface receptors in the gate of CD56⁺ NK cell populations. In each panel, dark line: isotype control; blue/red line: stained sample. Data were repeated independently in three separate experiments. D–K Violin plots of cytokine secretion levels in CTRL(eGFP)-iNK cells and hnCD16FR-iNK cells. Cytokines release was assessed using the flow cytometry analysis. Data were repeated independently in three separate experiments. L–M ADCC assays using a standard luciferase-based bioluminescence assay. ADCC against the lymphoma cell line Raji with/without anti-CD20 mAb (L) and against the lung cancer cell line A549 with/without anti-EGFR mAb (M). Statistical significance at 16:1 E:T ratio was determined by two-way ANOVA. Results are representative of three independent experiments; p > 0.05 (ns), p ≤ 0.05 (*), p ≤ 0.01 (**), p ≤ 0.001 (***), p ≤ 0.0001 (****)