Fig. 1
From: GAS6-based CAR-T cells exhibit potent antitumor activity against pancreatic cancer
GAS6-CAR-T cells target cells overexpressing TAM members. A Flow cytometry analysis of TAM family (AXL, TYRO3, and MERTK) protein levels in various pancreatic cancer cell lines (PANC1, MIA PaCa2, BxPC3, and ASPC1), human embryonic kidney cell 293 T (HEK-293 T), ASPC1-gemcitabine-resistant (ASPC1-Gem) cells, and cell line-derived cancer stem cells (PANC1-CSC and MIA PaCa2-CSC). B The level of TAM protein overexpression in HEK-293 T and BxPC3 cell lines was tested by western blot, and GAPDH was used as a loading control. Cytotoxicity of GAS6-CAR-T cells on TAM-overexpressing HEK-293 T (C) and BxPC3 (D) cells at an E/T ratio of 4:1 for 24 h (n = 3). Enzyme-linked immunosorbent assay (ELISA) was used to analyze IFN-γ (E& F) and TNF-α (G& H) release by either Mock T cells or GAS6-CAR-T cells in coculture supernatant