Fig. 4
From: GAS6-based CAR-T cells exhibit potent antitumor activity against pancreatic cancer
GAS6-CAR-T cells efficiently kill drug-resistant cells and cancer stem-like cells. A The levels of TAM proteins in ASPC1 and ASPC1-gemcitabine-resistant (ASPC1-Gem) cells were determined by western blot with GAPDH used as a loading control. B The cytotoxicity of GAS6-CAR-T cells on target cells was assessed an E/T ratio of 4:1 for 24 h (n = 3). ELISAs were used to detect IFN-γ (C) and TNF-α (D) release by T cells in coculture supernatants. E The levels of TAM proteins in parental cells (PANC1, MIA PaCa2) and cell line-derived cancer stem cells (PANC1-CSC and MIA PaCa2-CSC) were determined by western blot with GAPDH used as a loading control. The cytotoxicity of GAS6-CAR-T cells on luciferase-expressing target cells PANC1-CSC (F) and MIA PaCa2-CSC (G) was quantified at an E/T ratio of 4:1 for 24 h (n = 3). ELISAs were used to detect IFN-γ (H& I) and TNF-α (J&K) release by T cells in coculture supernatants