Fig. 8
From: GAS6-based CAR-T cells exhibit potent antitumor activity against pancreatic cancer
Safety of GAS6-CAR-T cells in nonhuman primate. A Transduction efficiency of GAS6-CAR into macaques T cells and non-transduced T (NT) cells was used as control. B DNA copies of GAS6-CAR-T cells in the peripheral blood of macaques were quantified by real-time PCR. GAS6-CAR-T cells (2 × 106 cells/kg) were autologously infused to rhesus macaques, and the peripheral blood was collected before (day 0) and after (days 1, 3, 5, 7, 14, 21, 28, and 35) injecting GAS6-CAR-T cells into rhesus macaques (n = 3). C Analysis of physiological indexes (diastolic and systolic blood pressure, heart rate, anus temperature, body weight). Biochemical indicators for D alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), γ-glutamine acylase (GGT), total protein (TP), albumin (ALB), globulin (GLO), ALB/GLO (A/G), total bilirubin (TBIL), E blood glucose (GLU), F blood urea nitrogen (BUN) and creatinine (CRE), G cholesterol (CHOL) and triacylglycerol (TRIG), H creatine kinase (CK) were tested. Blood routines for I red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), J platelet count (PLT), platelet distribution density (PDW), mean platelet volume (MPV), platelet volume ratio (PCT), K white blood cell (WBC), L monocyte, and M lymphocyte were analyzed