Fig. 5

Progressive tumor shrinkage in a 60-year-old metastatic colon carcinoma patient with confirmed PR evaluated on three index lesions. The patient underwent left hemicolectomy in 2018 with histological evidence of moderately differentiated intestinal adenocarcinoma, RAS mutated. The patient was treated for stage IV disease with multiple regimens (FOLFOX plus Bevacizumab followed by surgical resection of liver metastases; FOLFIRI plus Aflibercept, FOLFOX, FOLFIRI rechallenges, Trifluridine/Tipiracil, and Regorafenib). The patient had disease progression and was enrolled within cohort V (5 mg/kg for four consecutive days). The RECIST 1.1 assessment after the first cycle with LNA-i-miR-221 showed a clinical benefit. The patient received three subsequent treatment cycles on a compassionate use basis (pre-planned in the study protocol for patients with clinical response). The CT-image shown in the Figure demonstrates disease regression at cycle 1 with deepening response after cycle 4. Further tumor regression of the residual liver lesion has been observed after 7 months of follow-up. At the present (May 2023) the patient is still alive with PS 0