Fig. 2
From: CRISPR screening in hematology research: from bulk to single-cell level
Overview of CRISPR screening with readout at bulk or single-cell level. After delivery of the gRNA library, transduced cells are enriched and undergo CRISPR editing. Perturbed cells are subjected to a selective pressure to reveal enrichment or depletion of certain sequences, which are quantified and ranked in bulk CRISPR screening. Alternatively, cells can be subjected to single-cell sequencing, revealing the transcriptomic (or multi-omic) signatures per perturbation