Fig. 4
From: CRISPR screening in hematology research: from bulk to single-cell level
New applications of scCRISPR. a Different types of gRNA libraries can be used for different purposes. Multiplexed libraries target either a single gene by multiple gRNAs for highly efficient targeting, or multiple genes in a single cell to assess combinatorial effects. Base editing or tiling screens induce intragenic edits, while overexpression libraries ectopically introduce coding sequences. b Libraries can be delivered in vivo or in vitro, with high or low multiplicity of infection. c Multi-omic readouts, including transcriptomic, epigenomic or proteomic signatures, can be generated for each single cell (CDS = coding sequence, var = coding variant, HDR = homology directed repair, Tn5 = Tn5 transposase)