Figure 3

Ras inhibition affects erythroid proliferation and differentiation in vitro. A). Total colony counts of sorted GFP bone marrow cells from N17 Ras and control GFP mice. The same number of cells was plated in methylcellulose media in the presence of Epo. Colonies were counted 2 and 5 days after plating for BFU-Es and CFU-Es respectively. Results revealed that the growth of bone marrow cells is affected by Ras inhibition. B) The number of BFU-E and CFU-E colonies derived from GFP sorted cells from N17 Ras and control GFP mice were quantified as the percentage of total number of erythroid colonies present for each group at day 5. Results are shown as the mean ± standard deviation of two independent experiments.