Figure 6
Immunoprecipitation and western-blot analysis of heterodimerization interaction by TW-37 between anti-apoptosis and pro-apoptosis Bcl-2 family proteins. WSU-FSCCL cells were treated with 1 or 2 μM of TW-37 for 24 hr, lysed and 300 μg of whole cell lysate was immunoprecipitated with anti-Bim followed by Western-Blot with anti-Mcl-1, anti-Bcl-XL, anti-Bim and anti β-actin.