Figure 4

Generation of gp96 knockdown RPMI-8226 cell line. (A) Immunoblot analysis of gp96 after stable transduction with empty vector (EV) or gp96 shRNA lentiviral vector (gp96 KD). (B) Loss of surface integrins expression (open histogram) by flow cytometry in gp96 KD cells. Shaded histograms represent background staining with isotype-control antibody. THP-1 cells were used for positive control for this experiment.