Dysregulation of miR-15a and miR-214 in human pancreatic cancer

Table 2 Target validation for miR-15a

miR-15a target		Synthesized 3'-UTR containing the predicted MRE	MRE validated by luciferase activity	Specifically suppressed by miR-15a mimics
PIM1	F	TCGAGTACTTGAACTTGCCTCTTTTACCTGCTGCTTCTCCAAAAATCTGCCTGGGTTGC	YES	NT
	R	GGCCGCAACCCAGGCAGATTTTTGGAGAAGCAGCAGGTAAAAGAGGCAAGTTCAAGTAC
CDC25A	F	TCGAGGAGTAGAGAAGTTACACAGAAATGCTGCTGGCCAAATAGCAAAGACAACCTGGC	YES	NT
	R	GGCCGCCAGGTTGTCTTTGCTATTTGGCCAGCAGCATTTCTGTGTAACTTCTCTACTCC
BCL2L2	F	TCGAGGATTTTATTTGCATTAAGGGGTTTGCTGCTGAAAAAAAGTTGGAAAACCACTGC	YES	NT
	R	GGCCGCAGTGGTTTTCCAACTTTTTTTCAGCAGCAAACCCCTTAATGCAAATAAAATCC
WNT3A	F	TCGAGCGTTTTTGGTTTTAATGTTATATCTGATGCTGCTATATCCACTGTCCAACGGGC	YES	YES
	R	GGCCGCCCGTTGGACAGTGGATATAGCAGCATCAGATATAACATTAAAACCAAAAACGC
SMAD7	F	TCGAGCAGGCCACACTTCAAACTACTTTGCTGCTAATATTTTCCTCCTGAGTGCTTGGC	YES	NT
	R	GGCCGCCAAGCACTCAGGAGGAAAATATTAGCAGCAAAGTAGTTTGAAGTGTGGCCTGC
LRP6	F	TCGAGTATATATTTTCTTAAAACAGCAGATTTGCTGCTTGTGCCATAAAAGTTTGTAGC	YES	NT
	R	GGCCGCTACAAACTTTTATGGCACAAGCAGCAAATCTGCTGTTTTAAGAAAATATATAC
FGF7	F	TCGAGTATTCCTATCTGCTTATAAAATGGCTGCTATAATAATAATAATACAGATGTTGC	YES	YES
	R	GGCCGCAACATCTGTATTATTATTATTATAGCAGCCATTTTATAAGCAGATAGGAATAC

The 59-bp segments of the 3'-UTR of each target gene are listed in this table. F (forward sequence) and R (reverse sequence) were annealed together and inserted into the psi-CHECK-control vector. NT, negative.

ISSN: 1756-8722