Table 4 qRT-PCR Primers for miRNAs and U6
From: Dysregulation of miR-15a and miR-214 in human pancreatic cancer
miRNA | Primer name | Primer sequence (5' to 3') |
|---|---|---|
miR-15a | RT-primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGAC CACAAAC |
| Â | QF | GCGGCTAGCAGCACATAATGG |
miR-27a | RT-primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGAC GCGGAAC |
| Â | QF | GCGGCTTCACAGTGGCTAAGT |
miR-100 | RT-primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGAC CACAAGT |
| Â | QF | GCGGCAACCCGTAGATCCGAA |
miR-125b | RT-primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCACAAG |
| Â | QF | GCGGCTCCCTGAGACCCTAAC |
miR-181 | RT-primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGAC ACTCACC |
| Â | QF | GCGGCAACATTCAACGCTGTC |
miR-200a | RT-primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGAC ACATCGT |
| Â | QF | GCGGCTAACACTGTCTGGTAA |
miR-214 | RT-primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGAC ACTGCCT |
| Â | QF | GCGGCACAGCAGGCACAGACA |
miRNA | QR | GTGCAGGGTCCGAGGT |
U6 | U6QF | CTCGCTTCGGCAGCACA |
| Â | U6QR | AACGCTTCACGAATTTGCGT |
All primers are listed in this table. The RT-primer was used for the reverse transcriptase reaction. QF and QR were used for the PCR reaction. QR was applied to each miRNA test. U6QF and U6QR were used for examination of the U6 gene. | ||