Figure 10

Overexpression of Sall4 immortalized 32D cells and blocked G-CSF dependent differentiation. (a)Wright-Giemsa staining of 32D cells shows morphology of 32D cells with IL-3 alone (i), with G-CSF alone (ii), or transduced with Sall4A and with G-CSF alone (iii). (b) Growth curves of Sall4-32D cells cultured with G-CSF alone. In cells that were transduced with Sall4A or Sall4B, a 5 or 4-fold increase in the number of viable cells was observed from day 1 to 6.